Optimization of Reflux Extraction for Total Lignans from Acanthopanax Sessiliflorus by Orthogonal Experi-ment
- VernacularTitle:回流提取无梗五加根总木脂素的正交试验研究
- Author:
Yang SONG
;
Xiaohong LI
- Publication Type:Journal Article
- Keywords:
Acanthopanax sessiliflorus;
Total lignans;
Sesamin;
Orthogonal design;
UV spectrophotometry
- From:
China Pharmacist
2014;(5):748-750
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the optimal process of reflux extraction for lignans from Acanthopanax sessiliflorus roots. Methods:Sesamin as the reference substance, a colorimetric method was used to detect the absorption value of the samples at 558 nm to calculate the content directly. The extraction time, the extraction times and the volume of extraction solvent were applied to optimize the extrac-tion conditions for total lignans by orthogonal experiment. Results:The absorbance had a linear relationship with the amourt of sesamin within the range of 10. 6-53. 0 μg·ml-1(r=0. 999 7). The average recovery of sesamin was 102. 2%(RSD=1. 6%, n=6). The content of total lignans (measurement by sesamin) in the extracts was 10. 0 mg·g-1. The optimum reflux extraction conditions were as follows:adding 40-fold 50 % methanol, extracting once for 30 min. Conclusion:The UV method for determining the total lignans is feasible, stable and reliable as well as precise, and the optimal extraction process is reasonable.
