Effects of Artesunate on K562, K562/ADM Cell Apoptosis and NF-κB p65 Expression in Human Leukemia
- VernacularTitle:青蒿琥酯对人白血病 K562，K562/ADM细胞凋亡的作用及对NF-κB p65表达的影响
- Author:
Weiqin WANG
;
Hong YIN
- Publication Type:Journal Article
- Keywords:
Artesunate;
K562;
P65;
Apoptosis
- From:
China Pharmacist
2014;(5):729-731
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of artesunate on K562, K562 /ADM cell apoptosis and NF-κB p65 expression in hu-man leukemia. Methods: The effect of artesunate with different concentrations on K562 and K562 /ADM cell apoptosis at various times was studied by a flow cytometer. The effect of artesunate with 15 μmol·L-1 on NF-κB P65 expression in K562 was explored u-sing a Western blot method. Results:Artesunate showed little effect on K562 cell apoptosis, while exhibited notable effect on K562 /ADM cell apoptosis. K562 /ADM cells were more sensitive than K562 to artesunate at the concentration of 7. 5 μmol·L-1 and 15μmol·L-1(P<0. 01), and after 4-hour treatment with 15 μmol·L-1 artesunate, K562 /ADM cell apoptosis was much higher than K562(P<0. 01). Artesunate could reduce leukemia cell p65 expression at the concentration of 15 μmol·L-1. Conclusion:Artesu-nate can induce the apoptosis of leukemia cells by down-regulating the expression of p65.
