Effect of Fasudil on Inflammatory Secretion of Astrocytes
10.3870/j.issn.1672-0741.2014.02.022
- VernacularTitle:法舒地尔对星形胶质细胞炎性分泌的影响
- Author:
Zhihui DUAN
;
Congmin MA
- Publication Type:Journal Article
- Keywords:
Fasudil;
astrocyte;
TNF-α;
IL-8
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2014;(2):219-222
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Fasudil on inflammatory factors (TNF-α and IL-8) secreted by astro-cytes.Methods Primary rat spinal astrocytes were cultured and randomly divided into three groups :control group ,oxygen-glu-cose deprivation group (OGD group) and Fasudil group.The culture media were harvested at different time points (0 ,3 ,6 ,12 , 24 h) for detection of the concentration of TNF-αand IL-8 by ELISA.Results Compared with the control group ,the concentra-tions of TNF-αand IL-8 in OGD group were significantly increased at each time point (P<0.05);in Fasudil group ,the concen-trations of TNF-αand IL-8 increased at some time points ,but they were much lower than in OGD group (P<0.05) .Conclusion OGD can promote the secretion of TNF-αand IL-8 by astrocytes in vitro ,which can be effectively inhibited by Fasudil.It is suggested that Fasudil plays an important role in regulating the levels of TNF-αand IL-8 secreted by astrocytes.
