Study on UPLC-MS Fingerprint of Bupleurum Marginatum in Northwest Hubei
- VernacularTitle:鄂西北地区竹叶柴胡UPLC-MS指纹图谱研究
- Author:
Hua CAI
;
Fang YE
;
Guangyi YANG
;
Ling XIAO
;
Shiming DU
- Publication Type:Journal Article
- Keywords:
Bupleurum marginatum;
Fingerprint;
Quality control;
UPLC-MS
- From:
China Pharmacist
2014;(5):797-800
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the UPLC-MS fingerprint of Bupleurum marginatum DC. in northwest Hubei and establish the quality evaluation system for the herb. Methods:A UPLC-MS method was used to analyze 10 samples of B. marginatum DC with the following chromatographic conditions:an ACQUITY UPLC? RBEH C18 column (100 mm × 2. 1 mm, 1. 7 μm) was used, the mobile phase consisted of acetonitrile-0. 3% formic acid water with gradient elution, the flow rate was 0. 2 ml·min-1, and the detection wavelength was 203nm with ESI(-). Results:There were 8 common peaks in the UPLC-MS fingerprint of B. marginatum DC. Through analyzing the information of mass spectrometry and combining the references, 6 peaks were identified. Conclusion:The UPLC-MS fin-gerprint method is simple,rapid and feasible. The acquired UPLC-MS fingerprint of B. marginatum DC. and the evaluation indices can provide the scientific quality assessment of B. marginatum DC.
