Effect of Buzhong Yiqi Decoction serum on cell cycle of A 549/DDP cell
- VernacularTitle:补中益气汤含药血清对A549/DDP细胞生长周期作用的影响
- Author:
Huan JING
;
Ying TANG
;
Dan YU
;
Chunying LIU
- Publication Type:Journal Article
- Keywords:
Buzhong Yiqi Decoction;
A 549/DDP cell;
cell cycle;
apoptosis
- From:
Chinese Journal of Biochemical Pharmaceutics
2014;(2):48-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Buzhong Yiqi Decoction serum on cell cycle of A549/DDP cell. Methods A549/DDP cells and splenocytes cells were cultured in vitro and divided randomly into three groups:control group, Buzhong Yiqi Decoction group and splenocyte group. A 549/DDP cells were stimulated by different concentrations of Buzhong Yiqi Decoction serum and spleen cells, the direct killing effect on cells were measured by MTT method, cell apoptosis were detected by lfow cytometric analysis and the apoptotic body were observed by immunolfuorescence method. Results MTT results showed that cell growth were inhibited by Buzhong Yiqi Decoction serum in contrast with control groups, and the effect was same as spleen cells, and both treated groups had similar effects. Marked apoptotic peak and the apoptotic body were seen by lfow cytometric analysis and lfuorescence microscope in both two groups. Conclusion Buzhong Yiqi Decoction could inhibit the proliferation of A549/DDP cells in vitro and induced cell apoptosis, which is the similar as spleen cells.
