The effect of Chinese herbal compound Yitangkang extract on expression of renal AGEs and RAGEs in rats with diabetic nephropathy
- VernacularTitle:中药复方益糖康对DN模型大鼠肾脏组织中AGEs、RAGEs影响的实验研究
- Author:
Wenshun ZHANG
;
Yan SHI
- Publication Type:Journal Article
- Keywords:
diabetic nephropathy;
advanced glyclation end-products;
receptor of advanced glyclation end-products;
Yitangkang
- From:
Chinese Journal of Biochemical Pharmaceutics
2014;(2):45-47
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Chinese herbal compound Yitangkang on the expressions of advanced glyclation end-products (AGEs) and the receptor of advanced glyclation end-products mRNA(RAGE-mRNA) in rats with diabetic nephropathy(DN),and to discuss the mechanism of the formulas in the treatment of DN. Methods The rat model of DN were established by intraperitoneal injection of strepotozotocin(STZ). After 8 weeks intervention with medications, the expression of renal RAGEs mRNA was detected by Real-time PCR, renal RAGE by immunohistochemistry, and renal content of AGEs by spectrophotometry. Results Compared with normal control group, the expression of renal RAGEs mRNA and RAGEs in diabetic nephropathy rats were increased significantly(P<0.01). Compared with DN group, the expression of renal RAGEs mRNA and RAGEs in three doses Yitangkang groups and western medicine group were decreased significantly(P<0.01). Conclusion Chinese herbal compound Yitangkang could protect kidney of diabetic nephropathy rats,and the mechanism is related to its loweirng renal expression of RAGEs mRNA, RAGEs and AGEs content.
