Bioactivity and stability study of the antifungal substance produced by Streptomyces NG-715
- VernacularTitle:链霉菌NG-715的抗真菌活性及稳定性研究
- Author:
Min YUAN
;
Hua BIAN
;
Jing LI
;
Xuepeng ZHANG
;
Lin CHENG
- Publication Type:Journal Article
- Keywords:
Streptomyces;
antifungal substance;
minimum inhibitory concentration;
minimum bactericidal concentration;
stability
- From:
Chinese Journal of Biochemical Pharmaceutics
2014;(2):37-39
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the bioactivity and stability of the antifungal substance produced by Streptomyces NG-715 as well as to establish the assay for biological activity detection. Method Take the antifungal substance as experimental materials, and test its minimum inhibitory concentration and minimum bactericidal concentration on four fungi strains including Saccharomyces cerevisiae, Penicillium citrinum, Aspergillus niger, Rhizopus sp. Aspergillus niger was used as indicator strain to measure the biological activity and stability of the antifungal substance. Results The results showed that the MIC of the antifungal substance on four fungi strains including Saccharomyces cerevisiae,Penicillium citrinum,Aspergillus niger,Rhizopus sp were 1.25, 2.5, 3.75 and 3.75μg/mL, respectively. The MBC of the antifungal substance on four fungi strains were 2.5, 10, 17.5 and 17.5μg/mL, respectively. Linearity regress equation of the antifungal substance in Aspergillus niger was y=26.963 x-27.6,R 2=0.9991. The antifungal substance was pH-stable, heat-stable but ultravio1 et-sensible. Conclusion The results from this study will porvide useful information for the further extraction and analysis on the bioactive compound.
