Effects of Haizao Yuhu Decoction on chemokine receptor CXCR 4 of TT cell tumor-bearing mice
- VernacularTitle:海藻玉壶汤对甲状腺癌荷瘤小鼠趋化因子受体CXCR4的影响研究
- Author:
Qinliang ZHANG
;
Qi GUAN
- Publication Type:Journal Article
- Keywords:
HaizaoYuhu Decoction;
CXCR 4;
TT cell;
BALB/c mice
- From:
Chinese Journal of Biochemical Pharmaceutics
2014;(2):42-44
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Haizao Yuhu Decoction on the CXCR 4 expression of TT cell tumor-bearing mice. Methods Culture the human thyroid TT cells in vitro, and inoculate them into 30 healthy mice BALB/C. Subsequently, Haizao Yuhu Decoction was given to the tumor-bearing mice. They are grouped into three groups, including tumor-bearing group (n=10), Haizao Yuhu Decoction high dosage group (n=10) and Haizao Yuhu Decoction low dosage group (n=10) to observe the tumor growth to calculate the anti-tumor rate. After the tumor has been reaped, immunohistochemical is adopted to test the expressions of chemotactic factor receptor-CXCR 4 on protein level. RT-PCR is used to test the expressions of CXCR 4 on genetic level. Results Comparing with the tumor-bearing group, HZYHD high-dosage group has decreased the expressions of CXCR 4 on both the protein and genetic levels, which is to certain degree dependent on concentration.Conclusion Haizao Yuhu Decoction could decrease the expression of CXCR 4.
