Detection of Bacterial Endotoxin in Hemocoagulase Atrox for Injection by Kinetic Turbidimetric Assay
- VernacularTitle:动态浊度法检查注射用血凝酶的细菌内毒素
- Author:
Guojin WANG
;
Gang CHEN
;
Juan ZHANG
;
Jiansong QU
;
Qingfen ZHU
- Publication Type:Journal Article
- Keywords:
Hemocoagulase atrox for injection;
Bacterial endotoxins;
Kinetic turbidimetric assay
- From:
China Pharmacist
2014;(4):684-685
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the criterion of bacterial endotoxins test for hemocoagulase atrox for injection and measure the endotoxin concentration in hemocoagulase atrox for injection by the kinetic turbidimetric technique. Methods:The limit of bacterial en-dotoxins in the product was designed according to ChP 2010. Four endotoxin concentrations were prepared to obtain the standard curve. The interfering test was done by measuring the concentration of endotoxin added into the sample solution. The endotoxin concentration in the sample solution was measured. Results:The absolute value of the correlation coefficient was 0. 988 6 (must be above or equal to 0. 980), suggesting the standard curve was valid. The recovery of the added endotoxin in the sample solution was within 50 to 200%when the concentration of the product sample was 0. 25 KU·ml-1 . The measured endotoxin concentration in the sample met the re-quirements for bacterial endotoxins. Conclusion:The bacterial endotoxins assay method for milrinone injections is established, and the kinetic turbidimetric technique is suitable for the bacterial endotoxin test of the product.
