Determination of Osthole and Columbianadin in Wangbi Tablets by HPLC
- VernacularTitle:HPLC法测定尪痹片中蛇床子素和二氢欧芹醇当归酸酯含量
- Author:
Leibing XUE
;
Yan ZHOU
;
Peifen JIN
- Publication Type:Journal Article
- Keywords:
Wangbi tablets;
Osthole;
Columbianadin;
HPLC
- From:
China Pharmacist
2014;(4):697-699
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the determination of osthole and columbianadin in Wangbi tablets. Meth-ods:The column was an Agilent XDB C18(150 mm ×4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-water(49∶51)with gradient elution. The flow rate was 1. 0 ml·min-1 and the detection wavelength was at 330 nm. Results: The calibration curve was linear within the range of 0. 060-0. 597 μg(r=0. 999 9) for osthole and 0. 010-0. 102 μg(r=0. 999 9) for columbianadin. The aver-age recovery was 99. 12% and 98. 98%, respectively. Conclusion:The method is simple, practicable, accurate and rapid. It can be applied in the content determination of osthole and columbianadin in Wangbi tablets.
