Alpha-lipoic acid pretreatments provide protection against hypoxia/reoxygenation injury in human umbilical vein endothelial cell
10.3969/j.issn.1006-5725.2014.07.014
- VernacularTitle:α-硫辛酸预处理对人脐静脉内皮细胞缺氧复氧损伤的保护作用
- Author:
Jingjing ZHANG
;
Houliang DENG
;
Aimin JI
- Publication Type:Journal Article
- Keywords:
Thioctic acid;
Hypoxia/reoxygenation;
Oxidative stress;
HUVEC;
Apoptosis
- From:
The Journal of Practical Medicine
2014;(7):1053-1056
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the protective effect of α-lipoic acid (LA) pretreatment on hypoxia/reoxyg-enation injury in vascular endothelial cells and preliminary explore its mechanism. Methods HUVEC were cultured intro and devided into control group,hypoxia/reoxygenation model group and LA group. Cell morphology was observed under inverted phase contrast microscope,cell viability was detected by MTT,total antioxidant capacity (TAOC),nitric oxide(NO),malondialdehyde (MDA),superoxide dismutase (SOD) activity or level were measured by various commercial kits respectively. Apoptosis of HUVEC in each group were detected by flow cytometry , cleaved caspase-3 level was measured by western blot. Results As compared to control group, the survival rate, TAOC, SOD, NO activity were significantly lower(P<0.05), and MDA level, apoptosis rate and apoptosis protein levels were significantly higher (P < 0.05) in model group. However,LA can increase the survival rate,TAOC, SOD,NO activity (P < 0.05),reduce MDA level and cell apoptosis (P < 0.05)in a dose dependent manner. Conclusion LA pretreatments provide protection against hypoxia/reoxygenation injury in HUVEC. LA exerts protective effects through inhibition of HUVEC apoptosis.
