VX-680 induces apoptosis of T24 human bladder cancer cells and down-regulates Bcl-2 expression
10.3969/j.issn.1006-5725.2014.07.016
- VernacularTitle:VX-680诱导人膀胱癌T24细胞凋亡及其对Bcl-2表达的影响
- Author:
Ning XIAO
;
Xing ZHOU
;
Gewa ZENG
;
Xiaokun ZHAO
- Publication Type:Journal Article
- Keywords:
Urinary bladder neoplasms;
VX-680;
Human bladder cancer T24 cell;
Apoptosis;
Bcl-2
- From:
The Journal of Practical Medicine
2014;(7):1060-1062
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of aurora kinase inhibitor VX-680 on cell apoptosis and Bcl-2 expressions in human bladder cancer T24 cells. Methods T24 cells were cultured and treated with VX-680 at various concentrations and time points in vitro. VX-680-induced apoptosis of T24 cells was calculated by flow cytometry. The morphological change of treated cells was observed by microscopy;Bcl-2 mRNA and protein expression in T24 cells was detected by RT-PCR and Western blot assay , respectively. Results The apoptosis rate of VX-680-induced T24 cells increased in a dose-and time-dependent manner. The increase of apoptosis rate and decrease of Bcl-2 mRNA and protein expression in VX-680-induced T24 cells were in a dose-dependent manner. Conclusion VX-680 can significantly induce the apoptosis of T24 cells by down-regulating Bcl-2expression in a dose-dependent manner.
