Cloning and prokaryotic expression of human Cyclin D1 gene

VernacularTitle:人 Cyclin D1基因克隆与原核表达
Author: Yan TAN ; Xiaofang HAN ; Haiqin JIA ; Ruixia BAI
Publication Type:Journal Article
Keywords: Liver neoplasms/genetics; Cyclin D1; Cloning,molecular; Gene expression
From: Journal of Chinese Physician 2014;(2):224-226
CountryChina
Language:Chinese
Abstract: Objective To obtain the Cyclin D1 through cloning and prokaryotic expression of Cyclin D 1 gene.Methods The total RNA was extracted from liver cancer tissue .The Cyclin D1 cDNA was obtained by reverse transcriptase polymerase chain reaction (RT-PCR).The Cyclin D1 cDNA was sequenced, and sub-cloned to the PET32a+.The prokaryotic expressed was used to obtain the Cyclin D1.Results The 483 bp Cyclin D1 cDNA was obtained.The sequence of Cyclin D1 was corrected.The 36 KD CyclinD1 was obtained by prokaryotic expression .Conclusions The Cyclin D1 cDNA was obtained.Cyclin D1 was expressed in BL21.