Osteogenic differentiation induced by bone morphogenetic protein 2 and long non-coding RNA AK007000
10.3969/j.issn.2095-4344.2014.15.001
- VernacularTitle:骨形态发生蛋白2诱导成骨分化与长链非编码RNA AK007000的影响
- Author:
Yan GAO
;
Chen CHENG
;
Jing LI
;
Yue ZHANG
;
Weifan XIAO
;
Qiuhui PAN
- Publication Type:Journal Article
- Keywords:
bone morphogenetic proteins;
alkaline phosphatase;
osteocalcin;
RNA interference
- From:
Chinese Journal of Tissue Engineering Research
2014;(15):2297-2302
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Long non-coding RNA (lncRNA) regulates a series of physiological processes and it is considered to play important roles in the gene regulation of development, differentiation and metabolism. MC3T3-E1, C2C12 and C3H10T1/2 cells are able to differentiate into different celllineages, such as bone cells and muscle cells, and they can be used in the study of musculoskeletal diseases.
OBJECTIVE:To study the role of lncRNA in osteogenic differentiation induced by bone morphogenetic protein 2.
METHODS:Osteogenic differentiation of MC3T3-E1, C2C12 and C3H10T1/2 cells was induced by bone morphogenetic protein 2, and microarray expression profiling of lncRNA was undertaken in osteogenic differentiation. LncRNA simultaneous changes in three cells were found out. The siRNA interference of the lncRNA was used to study its effects on the osteogenic differentiation induced by bone morphogenetic protein 2. Real-time PCR and alkaline phosphatase staining were applied to detect osteogenesis related indicators.
RESULTS AND CONCLUSION:In the process of osteogenic differentiation induced by bone morphogenetic protein 2, osteogenic differentiation indicators were increased, while myogenic differentiation indicator myogenin was reduced. LncRNA AK007000 was screened out to play a role in osteogenic differentiation induced by bone morphogenetic protein 2. Knockdown of lncRNA AK007000 decreased the expression of osteogenic differentiation indicators, while increased the expression of myogenin. Therefore, AK007000 may play a role in promoting osteogenic differentiation and inhibiting myogenic differentiation.
