Effects of non-dextran coated superparamagnetic iron oxide nanoparticles on proliferation of bone marrow mesenchymal stem cells
10.3969/j.issn.2095-4344.2014.16.011
- VernacularTitle:无葡聚糖包被超顺磁性氧化铁纳米颗粒影响骨髓间充质干细胞的增殖
- Author:
Peng CHEN
;
Jie ZHANG
;
Dongming RONG
;
Zhongyu HAN
;
Sijie YUAN
;
Jing TIAN
- Publication Type:Journal Article
- Keywords:
biocompatible materials;
magnetite nanoparticles;
stem cels;
cellproliferation;
lactate dehydrogenases
- From:
Chinese Journal of Tissue Engineering Research
2014;(16):2526-2531
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Currently, the research about effect of non-dextran coated superparamagnetic iron oxide nanoparticles on cellproliferation and cytotoxicity is relatively much less. OBJECTIVE:To evaluate the effects of 0, 25, 50, 75, 100 mg/L non-dextran coated superparamagnetic iron oxide nanoparticles on the proliferation and cytotoxicity of rat bone marrow mesenchymal stem cels. METHODS:Culture media containing 0, 25, 50, 75, 100 mg/L non-dextran coated superparamagnetic iron oxide nanoparticles were prepared for culture of bone marrow mesenchymal stem cels. After 24 hours of culture, the cels were confirmed using Prussian blue staining, and cellcounting was detected using cellcounting kit-8. Meanwhile, lactate dehydrogenase activity in the supernatant and intracelular superoxide dismutase activity were detected. RESULTS AND CONCLUSION:Loading of non-dextran coated superparamagnetic iron oxide nanoparticles in BMSCs was confirmed by Prussian blue staining. The percentage of cels labeled with non-dextran coated superparamagnetic iron oxide nanoparticles was up to 100% when the cels were incubated with a non-dextran coated superparamagnetic iron oxide nanoparticle solution of 50 mg/L and above, but 25 mg/L was insufficient to label al of the cels. Furthermore, as the concentration of non-dextran coated superparamagnetic iron oxide nanoparticles decreased, the cellproliferation rate decreased gradualy. The 25 mg/L group had a minimum cellproliferation rate, but the 25 and 50 mg/L groups showed no statisticaly significant difference (P > 0.05). Therefore, 50 mg/L is considered as the appropriate concentration of non-dextran coated superparamagnetic iron oxide nanoparticles, under which, the labeling efficiency is higher and the cytotoxicity is lower.
