Antibody preparation and enzyme linked immunosorbent assay of Lonicera japonica phenylalnine ammonia lyase.
- Author:
Zhouyong WANG
;
Luqi HUANG
;
Yuan YUAN
;
Baomin WANG
- Publication Type:Journal Article
- From:
Acta Pharmaceutica Sinica
2013;48(9):1498-502
- CountryChina
- Language:Chinese
-
Abstract:
The expression of phenylalnine ammonia lyase (LJPAL1) is closely related to the content of active compounds in Lonicera japonica. In this paper, a prokaryotic expression vector is constructed and LJPAL1 protein is expressed in E. coli. Three antigen sites were synthesized to peptide antigen and prepared polyclonal antibody of Anti-LJT-1, Anti-LJT-2 and Anti-LJT-3, separately. Antibody Anti-LJT-2 was screened using Western blotting. And indirect ELISA was built using Anti-LJT-2. The results of this study will be a base for honeysuckle chemical quality and evaluation kits.
