Correlation between Bmi-1 and clinicopathological features of colorectal cancer
10.3969/j.issn.2095-4344.2014.06.012
- VernacularTitle:大肠癌干细胞基因Bmi-1表达与大肠肿瘤组织病理特征的关系
- Author:
Zhe HUANG
- Publication Type:Journal Article
- Keywords:
intestine,large;
diagnosis,differential;
genes;
stem cells;
tissues
- From:
Chinese Journal of Tissue Engineering Research
2014;(6):894-899
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Bmi-1 (B-cel-specific moloney murineleukemia virus insertion site), a colorectal cancer stem cellgene is of great significance in the regulation of telomere reverse transcriptase activity and transcriptional status as wel as cellaging and carcinogenesis.
OBJECTIVE:To investigate the correlation between Bmi-1 expression and clinicopathological changes of colorectal cancer.
METHODS:The expression of Bmi-1 mRNA in carcinoma cells was detected in 50 cases of colorectal cancer using quantitative real-time PCR. The expression of Bmi-1 protein in cancer tissue was detected by hematoxylin-eosin staining and immunohistochemical staining SP method.
RESULTS AND CONCLUSION:Sex, age, and tumor size showed no different effects on the expression of Bmi-1 mRNA. The expression of Bmi-1 protein showed no significant difference in patients who were different in sex, age, tumor size and Duke staging of tumor. The expressions of Bmi-1 mRNA and protein in patients with lymph node metastasis and depth of invasion deeper than the serosa were much higher than those in patients with no lymph node metastasis and depth of invasion shal ower than the serosa (P<0.05). The expression of Bmi-1 mRNA was much higher in patients with higher Duke staging cancer (P<0.05). The expression of Bmi-1 mRNA and protein are closely related to different pathological features of colorectal cancer, such as metastasis, infiltration, and Duke staging.
