Single-center comparative analysis of panel reactive antibodies of 280 cases detected by two methods
10.3969/j.issn.2095-4344.2014.05.019
- VernacularTitle:单中心两种方法检测280例群体反应性抗体的对比分析
- Author:
Guojie KUANG
;
Hongwen WU
;
Wenqiang ZHOU
;
Li XIAO
- Publication Type:Journal Article
- Keywords:
kidney transplantation;
enzyme-linked immunosorbent assay;
microchip analytical procedures;
HLA antigens
- From:
Chinese Journal of Tissue Engineering Research
2014;(5):767-772
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Liquid chip techniques (Luminex) is a recently rising method for detecting anti-panel reactive antibody, which is characterized by high sensitivity, and strong specificity, less interference and high flux.
OBJECTIVE:To compare the sensitivity and detection difference of panel reactive antibody in serum of kidney disease patients detected by enzyme-linked immunosorbent assay and Luminex.
METHODS:Serum samples of 280 patients with kidney disease were selected. The enzyme-linked immunosorbent assay and Luminex methods were used to measure positive rate of panel reactive antibody. Chi-square test for fourfold table data was utilized for statistical analysis.
RESULTS AND CONCLUSION:The positive rates of panel reactive antibody were respectively 18.9%and 33.6%as detected by enzyme-linked immunosorbent assay and Luminex method. The positive rates of anti-HLA-I antibody and anti-HLA-II antibody were respectively 12.8%and 12.5%, as detected by enzyme-linked immunosorbent assay. The positive rates of anti-HLA-I antibody and anti-HLA-II antibody were respectively 25.0%and 20.7%, as detected by Luminex method. Positive detection rate was significantly higher in the Luminex group than that in the enzyme-linked immunosorbent assay group. Moreover, Luminex method could precisely detect the low-concentration antibody. Chi-square test for fourfold table data showed P<0.01. Significant differences in the differences of panel reactive antibody of kidney disease patients were detected between the two methods. Results demonstrated that compared with enzyme-linked immunosorbent assay, Luminex method is more sensitive and accurate, and more suitable for clinical detection.
