Discussion of the application of enzymatic reference methods in external quality assessment
10.3969/j.issn.1671-8348.2014.06.020
- VernacularTitle:探讨酶学参考方法在室间质评中的应用
- Author:
Yun LI
;
Xianzhang HUANG
;
Songbai ZHENG
;
Liqiao HAN
;
Yongjian CAO
;
Jianhua XU
;
Li LIN
;
Youqiang LI
;
Junhua ZHUANG
;
Jianbing WANG
- Publication Type:Journal Article
- Keywords:
enzyme;
reference method;
external quality assessment;
clinical analytic system;
mutual recognition of test results
- From:
Chongqing Medicine
2014;(6):692-695
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the feasibility of enzymatic reference methods in Routine Chemistry external quality assessment (EQA)inlaboratorymedicine.Methods Samplesofthe1stEQAin2012byNationalCenterforClinicalLaboratories(NCCL)and patients′sera were measured by reference methods and 5 clinical analytic systems for the catalytic activity of CK ,LDH ,ALP ,ALT , AST ,GGT and AMY ,then the results of 5 clinical systems were compared with the reference methods′or target value of NCCL by calculating the bias ,and evaluated them according to the criteria of EQA by NCCL .Results The results of EQA samples measured by reference methods was within ± 10% compared with NCCL target value .Compared with the results of reference method ,the through put was 100 .0% for wet clinical chemistry systems measuring both EQA samples and patients′serum ,and the dry clinical chemistry systems was 77 .1 for EQA samples and 97 .1% for patients′serum according to the criterion of EQA ,and the through put was 72 .9% and 63 .6% of wet clinical chemistry systems according to the standard of enzymatic trueness of NCCL .Conclusion Reference method could be applied to EQA ,and will be a great help for the trueness of clinical testing .
