Establishment of human pancreatic tumor xenograft mouse model for evaluating tumor-homing and gene-silencing effects of siRNA-loading nanoparticles
10.3969/j.issn.1000-4718.2014.03.034
- VernacularTitle:人胰腺癌 PANC-1细胞裸鼠移植瘤模型建立及其用于观察载 siRNA 纳米微粒体内效应的研究
- Author:
Linjuan ZENG
;
Jingguo LI
;
Qiubo ZHANG
;
Chenchen QIAN
;
Zhong LIN
;
Yinting CHEN
;
Kaihong HUANG
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
Models,animal;
Nanoparticles;
RNA interference
- From:
Chinese Journal of Pathophysiology
2014;(3):572-576
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To establish an effective and rapid method to develop transplanted subcutaneous pancreatic carcinoma by inducing PANC-1 cells into nude mice, and then use this mouse model to evaluate the tumor-homing and gene-silencing effects of siRNA-loading nanoparticles in vivo.METHODS:Different numbers of PANC-1 cells in 100 μL or 300 μL PBS were inoculated subcutaneously into the right flank of BALB /c (nu/nu) mice.When the tumor volume reached 100 mm 3 , siRNACY 5.5 nanoparticles were injected through the mouse tail vein to perform in vivo imaging assay.Be-sides, the mice were randomly divided into 3 treatment groups treated with PBS, scrambled control RNA nanoparticles and siKras nanoparticles, respectively.The protein expression of Kras was detected by Western blotting and immunohistochemi-cal staining.RESULTS:After inoculated with 1 ×10 7 PANC-1 cells in 300 μL PBS, all mice developed tumors within 2 weeks.The in vivo results showed that siRNA-loading nanoparticles accumulated in the tumor tissues and exerted gene si-lencing effect.CONCLUSION:In the present study, an effective and rapid method was established for PANC-1 cells to induce transplanted subcutaneous pancreatic carcinoma in nude mice within 2 weeks, which is suitable for in vivo imaging and treatment evaluations as a reproducible and reliable way for the further experiments .
