Deoxyadenosine antagonized methotrexate-induced suppression of osteoclastogenesis
10.3969/j.issn.1001-3733.2014.02.06
- VernacularTitle:脱氧腺苷拮抗甲氨蝶呤对破骨细胞的抑制作用
- Author:
Pengfei QU
;
Haobo LI
;
Biao CHEN
;
Rui ZHANG
;
Zhiyu JIA
;
Yinghuai ZHANG
;
Quanyong TANG
- Publication Type:Journal Article
- Keywords:
Osteoclast;
Methotrexate(MTX);
Deoxyadenosine(dAdo);
Adenosine receptor(AR);
Caffeine(Ca);
Receptor activator ofnuclear factor kappa-B ligand(RANKL);
Osteoprotegerin(OPG)
- From:
Journal of Practical Stomatology
2014;(2):173-177
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the mechanism of the effectiveness loss of methotrexate(MTX)in the treatment of rheumatoid arthri-tis.Methods:The culture system of rat whole bone marrow cells(WBMCs)and tartrateresistant acid phosphatase(TRAP)staining were utilized to evaluate osteoclastogenesis.The mRNA expression of osteoclastogenesis factors in the WBMCs culture system was examined by semi-quantitative RT-PCR.Results:Deoxyadenosine(dAdo)decreased MTX-induced suppression of osteoclastogenesis.The recov-ery effect of dAdo on MTX was partially prevented by caffeine.MTX significantly reduced mRNA expression of receptor activator of nu-clear factor kappa-B ligand(RANKL),dAdo partially recovered RANKL mRNA expression and inhibited osteoprotegerin(OPG)expres-sion.Conclusion:The accumulation of dAdo may induce the effectiveness loss of methotrexate in rheumatoid arthritis treatment.Com-bination of MTX and caffeine can be a potential therapeutic strategy.
