The inhibition effects of Zyflamend on DMBA-induced oral carcinogenesis
10.3969/j.issn.1001-3733.2014.02.09
- VernacularTitle:Zyflamend 对实验性口腔癌抑制作用的研究
- Author:
Fang LI
;
Zheng SUN
;
Xuejin WANG
;
Xiaolin SUN
- Publication Type:Journal Article
- Keywords:
Zyflamend;
Oral cancer;
Cell proliferation;
Chemoprevention
- From:
Journal of Practical Stomatology
2014;(2):188-192
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of an unique anti-inflammatory herbal preparation Zyflamend(ZF)on 7,12-dimethyl-benz anthracene (DMBA)-induced oral carcinogenesis in golden Syrian hamsters.Methods:10 hamsters were used as the blank controls.106 hamsters were treated topically on the left cheek pouch with 0.5% DMBA solution,3 times per week for 6 weeks. Then the hamsters in positive control group(n =40)were not treated afterwards,in the other 2 groups were treated by topically ap-plication of ZF of original solution(high dose,n =33)and 1 ∶1 dilution(low dose,n =33)respectively.Hamsters were sacrificed at the 24th week and tissue samples of the left cheek pouch were obtained for the examination of tumor incidence,histopathology and BrdU immunohistochemisty.Results:Gross inspection showed that the incidences of the tumor in positive,low dose and high dose groups were 86.7%,53.6%(P <0.01 )and 50.0%(P <0.01 ),histopathological examination showed that the tumor incidences were 86.7%,78.6% and 64.3%,respectively(P <0.05).High dose of ZF significantly decreased the incidence of hyperplasia , dysplasia and cancer(P <0.05);decreased BrdU-labeling index(P <0.05).Conclusion:Zyflamend may inhibit DMBA induced oral carcinogenesis and such prevention may be related to the effect of the suppression of cell proliferation.
