Effect of Yiqi Yangyin Recipe and Its Different Assembling on Apoptosis Rate and Expression of Associated Protein in Acute Myeloid Leukemia Mice
10.3969/j.issn.1005-5304.2014.02.012
- VernacularTitle:益气养阴方及其拆方对急性髓细胞白血病小鼠细胞凋亡及相关蛋白的影响
- Author:
Ning JIAO
;
Chong CHEN
;
Ruirong XU
- Publication Type:Journal Article
- Keywords:
Yiqi Yangyin Recipe;
acute myeloid leukemia;
apoptosis;
protein expression;
mice
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(2):42-45
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Yiqi Yangyin Recipe (supplementing qi and nourishing yin) and its different assembling on apoptosis rate and associated protein expression of c-myc and survivin in mice, and explore its mechanism. Methods NOD-SCID mice were injected with l×106 KG1a cells from the tail vein after irradiation of 60Co. Model mice were randomly divided into control group, whole recipe group, strengthening body resistance group, eliminating pathogens group. The treatment groups were given correspongding administration, and control group was given equal volume of NS. Flow cytometry was applied to measure apoptosis rate. The immunochemistry method was used to detect the expression of c-myc, survivin. Results Apoptosis rate in all treatment groups were increased (P<0.01). The expression of c-myc and survivin in lymph node tissues in all treatment groups were significantly declined compared with control group (P<0.05). The effect of whole recipe group on the all indices was superior to strengthening body resistance group and eliminating pathogens group (P<0.05). Conclusion Yiqi Yangyin Recipe can decrease the expressions of c-myc and survivin, induce apoptosis in leukemia cells, therefore shows the therapeutic effect.
