Simultaneous Determination of Three Marker Components in Fufang Buwu Syrup by TLCS

VernacularTitle:TLCS同时测定复方补乌糖浆中3种指标成分含量
Author: Li CHEN ; Sheng WANG ; Xi WU ; Mei MENG ; Jingjing ZHU
Publication Type:Journal Article
Keywords: Fufang Buwu Syrup; TLCS; content determination
From: Chinese Journal of Information on Traditional Chinese Medicine 2014;(2):92-94
CountryChina
Language:Chinese
Abstract: Objective To establish an accurate method for determining the content of three components in Fufang Buwu Syrup. Methods TLC scanner was selected to detect three components with silica gel G thin layer plate. The sample was separated by using cyclohexane-ethyl acetate-methylenechloride-formic acid (3∶1∶1∶0.2),λS=300 nm. Results The linearity between peak area and ferulic acid was achieved in the range of 0.36-0.84μg, psoralen was achieved in the range of 0.12-0.28 μg, emodin was achieved in the range of 0.01-0.05 μg. The average recovery was 100.7%, 100.8%, 101.0%, and RSD was 1.26%, 1.44%, 1.86%, respectively. Conclusion The method is simple and accurate, which can be used for quality control of Fufang Buwu Syrup.