The effects of different titanium alloy surface topograph on the biological character of fibroblasts
10.3969/j.issn.1001-3733.2014.02.01
- VernacularTitle:不同钛合金表面形貌对成纤维细胞生物学行为的影响
- Author:
Wentian WANG
;
Weiyan MENG
;
Yanmin ZHOU
;
Qing CAI
;
Shuyan WANG
;
Jingjing WANG
;
Tongbin LIU
- Publication Type:Journal Article
- Keywords:
Electrochemical etching;
Titanium alloy;
Soft tissue;
Implant;
Micro-submicro-nano hierarchical structure
- From:
Journal of Practical Stomatology
2014;(2):151-155
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the effect of three different titanium alloy surface topographies on the biological character fibro-blasts.Methods:Three different titanium alloy Ti6Al4V surface topographies were created by machining(M),direct current electro-chemical etching(DC)and alternating current electrochemical etching(AC)respectively,and standard sized samples were prepared. The surface topographies were observed field emission scanning electron microscopy(FE-SEM).Mouse L929 fibroblasts were cultured on different surfaces.The attachment of cells was examined by acridine orange staining and observed under fluorescent microscope.The proliferation of cells was measured using MTT assay.Results:Tubles with the diamete of 5 -20 μm were observed on the surface of the sample of group DC,and more evenly distributed tubles on that of group AC.Micro-sulci were observed and no microtuble was found on the sample surface of group M.L929 cells were attached on the surface of all the samples in the 3 groups.More adhered and better strached cells were observed on AC treated surface.The proliferation of fibroblasts on AC and DC surfaces was superior to that on M treated surface and at the 3 -5 day the proliferation of fibroblasts on the AC surface was higher than that on DC treated surface.Con-clusion:AC treated surface with the micro-submicro-nano hierarchical topography of titanium alloy has a superior biocompatibility and can promote the early attachment and proliferation of the fibroblasts.
