Establishment of murine kidney transplantation models using both donor kidneys as grafts for two recipients
10.3760/cma.j.issn.0254-1785.2014.03.013
- VernacularTitle:同时利用双侧肾脏分别建立小鼠肾移植模型
- Author:
Jiangang HOU
;
Qiang DING
- Publication Type:Journal Article
- Keywords:
Mice;
Kidney transplantation;
Model
- From:
Chinese Journal of Organ Transplantation
2014;35(3):179-182
- CountryChina
- Language:Chinese
-
Abstract:
Objective Both kidneys of mice used To investigate the surgical procedures for establishment of murine kidney transplantation models by using bilaterial donor kidneys as grafts for two recipients.Method Using Balb/c male mice as donors/receptors,through careful dissection and trimming of donor renal vessels and abdominal great vessels,both right and left kidneys were harvested and separately transplanted into two recipients.The anastomosis between the aorta and vena cava of the graft and recipient mouse was made in end to-side manner using the 10-0 sutures.The artery and vein was anastomosed by dual point 180 continuous sutures.The technique of pulling the end of graft ureter into recipient's bladder and external fixation method were used for urethral reconstruction.Five days later the recipient's kidney was removed after transplantation.Result The kidneys in 30 mice were transplanted (15 left kidneys and 15 right kidneys) and model survival rate was 76.7％ (12/15 for the left and 11/15 for the right).The average operating time of donor was 339 min and that of recipient was 46 12 min.Cold ischemia time for the first and second transplantation was about 0.5 h and 1.5 h respectively.Conclusion Through careful microsurgical operation we establish the mouse kidney transplantation models using both donor kidneys as grafts,which reduces the costs of microsurgery experiments.
