Protective role of triptolide in a rat model of antibody-mediated rejection in kidney transplantation
10.3760/cma.j.issn.0254-1785.2014.03.012
- VernacularTitle:雷公藤内酯醇对大鼠肾移植抗体介导排斥反应的影响
- Author:
Gengwen HUANG
;
Xiang DING
;
Peng LEI
- Publication Type:Journal Article
- Keywords:
Triptolide;
Graft rejection;
Antibody specificity;
Kidney transplantation
- From:
Chinese Journal of Organ Transplantation
2014;35(3):175-178
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect and the mechanisms of triptolide on antibody-mediated rejection (AMR) in kidney transplantation.Method A rat model of AMR in kidney transplantation was constructed by sensitizing major histocompability complex (MHC) completely incompatible species of rats via blood transfusion before kidney transplantation.The protective effect of Triptolide on the renal function and pathologic injury was studied in this rat model.The levels of donor specific antibodies were measured by flow cytometry.C4d expression and macrophage infiltration were examined by immunohistochemistry and immunofluorescence.Result Triptolide could improve the function of transplanted kidney significantly (P＜0.05) and alleviate the pathologic injury.Triptolide can also reduce the levels of IgM,IgG2b and IgG2c in the serum of the recipient rats and inhibit the macrophage infiltration as well (P＜0.05).Conclusion Triptolide plays protective and therapeutic roles in AMR of kidney transplantation,which may be contributed to the inhibition of the production of donor specific antibody and the macrophage infiltration.
