Determination of hyperoside in Zhidai tablet by HPLC
10.3760/cma.j.issn.1673-4246.2014.02.011
- VernacularTitle:高效液相色谱法测定治带片中金丝桃苷的含量
- Author:
Zhihao LI
;
Xinjian SUN
;
Peng LI
- Publication Type:Journal Article
- Keywords:
Hyperoside;
Zhidai tablet;
HPLC
- From:
International Journal of Traditional Chinese Medicine
2014;36(2):135-137
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the determination of hyperoside in Zhidai tablet.Method The HPLC system used the Phenomenex LUNA-C18 (4.6 mm× 250 mm,5 μm)column,the mobile phase consisted of acetonitrile (10)-0.1％ H3PO4 (90),the flow rate was 1.0 ml/min,the column temperature was set at 30℃,the detection wavelength was set at 360 nm.Results The linear response range was 1.2～24.0 μg/mL(r=0.9998).The average recovery of mangiferin was 98.4％,and RSD was 0.93％.Conclusions The method is simple,rapid,accurate and repeatable.It can be applied in the determination of hyperoside in Zhidai tablet.
