Biocompatibility and osteoinductive activity of nano-hydroxyapatite/chitosan/poly(lactide-co-glycolide) scaffoldsin vitro
10.3969/j.issn.2095-4344.2014.08.009
- VernacularTitle:纳米羟基磷灰石/壳聚糖/聚丙交酯支架的体外生物相容性和成骨活性
- Author:
Fei WANG
;
Hong ZHOU
;
Yucheng GUO
;
Xiaoxia SU
;
Guozhou RAO
;
Xiaopeng ZHAO
- Publication Type:Journal Article
- Keywords:
biocompatible materials;
nanoparticles;
chitosan;
hydroxyapatites
- From:
Chinese Journal of Tissue Engineering Research
2014;(8):1198-1204
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Studies have found that combination of two of chitosan (CS), nano-hydroxyapatite (nHA) and poly(lactide-co-glycolide) (PLGA) can improve the mechanical properties and biocompatibility of the composite stent in certain extent as wel as improve osteogenic differentiation of the cels, but there is a certain distance from the ideal bone tissue engineering scaffolds.
OBJECTIVE:To study biocompatibility and osteoinductive activity of nHA/CS/PLGA scaffolds with different proportions in vitro.
METHODS: nHA/CS/PLGA scaffolds were prepared at mass ratio of 10:10:80, 10:20:70, 20:10:70 respectively by particle leaching method. And human bone marrow stem cels (hBMSCs) were co-cultured with these scaffolds in vitro. Adhesion, proliferation, and osteoinductive activity of these scaffolds were examined qualitatively and quantitatively by growth curve of hBMSCs on scaffolds. Gene expression of alkaline phosphatase activity and osteocalcin was detected by RT-PCR.
RESULTS AND CONCLUSION: hBMSCs could be attached, proliferated, and osteoinduced better on the nHA/CS/PLGA scaffold with the mass ratio of 20:10:70, compared to the other two groups of scaffolds. The differences were significant statisticaly (P< 0.05). Alkaline phosphatase and osteocalcin expressions were respectively higher in the scaffold with the mass ratio of 20:10:70 after 9-27 days of co-culture and 15-27 days of co-culture, in comparison with the other two groups of scaffolds. These findings indicate that the nHA/CS/PLGA scaffolds with the mass ratio of 20:10:70 demonstrated preferable biocompatibility and osteogenic inductivity, which is expected to be a promising scaffold material for bone tissue engineering.
