Extracelluar matrix stimulates neurite outgrowth of dorsal root ganglion neurons differently depending on whether extracted from degenerated or normal intervertebral disk
10.3969/j.issn.2095-4344.2014.07.010
- VernacularTitle:退变和健康椎间盘组织提取细胞外基质诱导背根神经节轴突生长的差异
- Author:
Hui ZHANG
;
Lin LIU
;
Wen XUE
- Publication Type:Journal Article
- Keywords:
intervertebral disk;
intervertebral disk degeneration;
nerve growth factor;
substance P
- From:
Chinese Journal of Tissue Engineering Research
2014;(7):1039-1044
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Compared with the normal intervertebral disk, the density of nerve fibers and number of nerve endings and neuropeptides appear to be more in the degenerated intervertebral disk. However, this phenomenon does not occur in the normal y aged disk.
OBJECTIVE:To evaluate the axonal growth and induction of a painful neuropeptide and substance P using rat dorsal root ganglion neurons and degenerated human disc cells in vitro.
METHODS:The human intervertebral discs were harvested from patients with discogenic low back pain and normal people. And extracelluar matrix extracted from human degenerative intervertebral discs was cultured with rat dorsal root ganglion neurons. The promotion of axonal growth and induction of substance P of dorsal root ganglion neurons in extracted medium were evaluated through morphology observation and enzyme-linked immunosorbent assay, respectively.
RESULTS AND CONCLUSION:Compared with the normal group, the content of nerve growth factor in the degenerative group was significantly higher and the average length of neuritis was significantly longer in the experimental group (P<0.05). After intervention with anti-nerve growth factorβ, the average length of neuritis became remarkably shorter. The percentage of substance P-immunoreactive cells was significantly higher in the degenerative group compared with the normal group (P<0.001). Nerve growth factors that highly express in the extracellular matrix from the degenerative intervertebral dick can promote neurite outgrowth of dorsal root ganglion neurons and induce release of neuropeptides related to pain transmission.
