COX-2 mRNA expression at different stages of osteoarthritis synoviocytes
10.3969/j.issn.2095-4344.2014.07.004
- VernacularTitle:不同分期骨关节炎滑膜细胞中COX-2 mRNA表达的差异
- Author:
Mingzhu ZENG
;
Kan DUAN
;
Changshen YUAN
;
Qijie MEI
;
Kai QIN
- Publication Type:Journal Article
- Keywords:
osteoarthritis,knee;
synovial membrane;
fibroblasts;
reverse transcriptase polymerase chain reaction;
COX-2
- From:
Chinese Journal of Tissue Engineering Research
2014;(7):1003-1008
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:COX-2 gene actual y exists in the joint fibroblast-like synoviocytes, it affects osteoarthritis occurrence and development. Understanding the differences of COX-2 gene expression levels at different stages of osteoarthritis synoviocytes has important theoretical significance for the occurrence and development of osteoarthritis, as wel as the role of synoviocytes in this process.
OBJECTIVE:To analyze the difference of COX-2 mRNA at different stages of osteoarthritis fibroblast-like synoviocytes.
METHODS:Synovial membrane from 44 osteoarthritis patients and 12 normal cases were selected. Primary cells were cultured to passage 4 fibroblast-like synoviocytes for the use in the experiment. COX-2 mRNA expression in osteoarthritis fibroblast-like synoviocytes and normal fibroblast-like synoviocytes was detected using real-time fluorescence quantitative RT-PCR. The relative quantitative analysis was performed using 2-ΔΔCt method.
RESULTS AND CONCLUSION:Expression of COX-2 mRNA in osteoarthritis fibroblast-like synoviocytes was significantly higher than that in normal fibroblast-like synoviocytes (P<0.05). The expression levels reached a peak at early osteoarthritis group, with significant differences compared with middle and late osteoarthritis groups (P<0.05). There was no significant difference between middle and later osteoarthritis groups (P>0.05). COX-2 mRNA might be important biological marker for the inflammation in osteoarthritis, and mainly plays a role in early osteoarthritis stage.
