Study of genetic susceptibility in 135 children with allergic asthma
10.3760/cma.j.issn.1673-4408.2014.02.022
- VernacularTitle:135例儿童过敏性哮喘遗传易患性研究
- Author:
Qian LI
;
Bin LUAN
;
Li SONG
;
Yanli ZHANG
- Publication Type:Journal Article
- Keywords:
Asthma;
Single nucleotide polymorphism;
Genetic susceptibility;
Children
- From:
International Journal of Pediatrics
2014;41(2):189-192
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the frequency distribution of single nucleotide polymorphisms (SNP) of four asthma-related gene loci in asthmatic children of Henan,and to investigate its association with genetic susceptibility to childhood asthma and some clinical phenotypes of asthma Methods Fluorogenic quantitative PCR and sequencing technique were employed to detect the frequency distributions of the SNP of the four asthma-related gene loci in 135 asthmatic children and 98 healthy controls.Results The genotype DD of angiotensin-converting enzyme (ACE)had a significantly higher frequency in the asthmatic children than in the healthy controls (x2 =26.475,P ＜ 0.01),and the frequency of D allele was also significantly higher in the asthmatic children than in the healthy controls (x2 =24.242,P ＜0.01).The genotype AG of Adrenaline receptor beta 2 subtypes (ADRB2) had a significantly higher frequency in the asthmatic children than in the healthy controls (x2 =22.505,P ＜0.01),and the frequency of allele was also significantly higher in the asthmatic children than in the healthy controls (x2 =6.759,P ＜ 0.01).Conclusions Genotype DD of ACE and genotype AG of ADRB2 are related to genetic susceptibility to childhood asthma and may be the risk factor for childhood asthma of Henan.Another two asthma genes involved in this study are not be able to repeat.
