Extraction technique of human genomic DNA from stools and its applications
10.3760/cma.j.issn.1009-9158.2014.01.017
- VernacularTitle:粪便中人基因组DNA提取技术及其应用
- Author:
Yong WANG
;
Bingjie ZOU
;
Guohua ZHOU
- Publication Type:Journal Article
- Keywords:
Feces;
DNA;
Genome,human;
Colorectal neoplasms;
Early diagnosis
- From:
Chinese Journal of Laboratory Medicine
2014;37(1):72-75
- CountryChina
- Language:Chinese
-
Abstract:
Noninvasive early diagnosis of colorectal cancer is conducive for patients to reduce the mortality rate and their suffering from diagnosis procedures.One of the most significant methods to proceed noninvasive early diagnosis of colorectal cancer is analysis of stool DNA.Extracting high quality and great quantity of human genomic DNA from stools guarantees diagnosis accuracy.However,the complexity of stool component hinders DNA extraction.Hence,it is crucial to develop highly efficient extraction methods of human genomic DNA from stools for the DNA analysis-based early diagnosis of colorectal cancer.Currently,two kinds of extraction strategies are employed:one is to directly extract total DNA from stools,the other is to enrich exfoliated colonocytes in stools before DNA extraction.This article reviews the advances on these two kinds of extraction techniques and summarizes their applications.
