Changes of the peripheral blood endothelial progenitor cells in peritoneal dialysis patients and its impact factors
10.3760/cma.j.issn.1001-7097.2014.02.004
- VernacularTitle:腹膜透析患者外周血内皮祖细胞变化及相关因素分析
- Author:
Xia YANG
;
Hong LEI
;
Jing YUAN
;
Shan HE
;
Yan SHEN
;
Yan ZHA
- Publication Type:Journal Article
- Keywords:
Peritoneal dialysis;
Stem cells;
Peripheral blood
- From:
Chinese Journal of Nephrology
2014;30(2):99-103
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the number and activities changes of peripheral blood endothelial progenitor cells (EPCs) in continuous ambulatory peritoneal dialysis (CAPD) patients,and explore the connection between EPCs' number and the levels of advanced glycosylation end products (AGEs),homocysteine (Hcy) and C-reactive protein (CRP).Methods Twenty-five CAPD patients and thirty healthy volunteers were involved.Total mononuclear cells (MNCs) were isolated from peripheral blood of patients.EPCs were characterized as adherent cells by double staining of FITCUEA-1 and DiL-AcLDL binding,and were further demonstrated by positive cells of CD34,CD133 and KDR using flow cytometry.The abilities of cell proliferation,adhesion and migration were further observed by fluorescent microscope.The correlations between the CEPCs' number and the levels of AGEs,Hcy and CRP were analyzed.Results The number and activities including migration and adhesion of EPCs in CAPD group were significantly lower than control group (P ＜ 0.05).The levels of serum AGEs,Hey and CRP in CAPD patients were increased (all P＜0.05) and had negative correlation with EPCs' number.Conclusions The number and activities of EPCs decrease in patients with CAPD,and EPCs' number is negatively correlated to the levels of AGEs,Hcy and CRP.
