Changes in expression of small intestinal thioredoxin 2 during different periods after orthotopic liver auto-transplantation in rats
10.3760/cma.j.issn.0254-1416.2013.11.006
- VernacularTitle:大鼠自体原位肝移植术后不同时期小肠组织硫氧还蛋白2表达的变化
- Author:
Mian GE
;
Xinjin CHI
;
Dezhao LIU
;
Gangjian LUO
;
Pinjie HUANG
;
Ailan ZHANG
;
Ziqing HEI
- Publication Type:Journal Article
- Keywords:
Liver transplantation;
Thioredoxins;
Intestine,small
- From:
Chinese Journal of Anesthesiology
2013;33(11):1315-1317
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the changes in the expression of small intestinal thioredoxin 2 (Trx2) during different periods after orthotopic liver autotransplantation (OLAT) in rats.Methods Forty Sprague-Dawley rats,aged 8-10 weeks,weighing 210-260 g,were randomly divided into 2 groups using a random number table:sham operation group (group S,n =8) and OLAT group (n =32).Intestinal tissues were removed at 4,8,16 and 24 h after OLAT for microscopic examination and for determination of the levels of superoxide anion (O2--),hydrogen peroxide (H2 O2),glutathione peroxidase (GSH-Px),reduced glutathione (GSH) and Trx2.Intestinal damage was assessed and scored according to Chiu.Results Compared with S group,the Chiu's score and O2--activity at 4,8 and 16 h after OLAT and H2O2 content at 4 and 8 h after OLAT were significantly increased,and the levels of GSH-Px and GSH and expression of Trx2 at 4 and 8 h after OLAT were decreased in OLAT group (P ＜ 0.05).Chiu' s score at 4,16 and 24 h after OLAT and H2O2 content at 16 and 24 h after OLAT were significantly lower than those at 8 h in OLAT group (P ＜ 0.05).Conclusion The rats undergo decreased antioxidant capacity in the early phase and recovery in the late phase mediated by small intestinal Trx2 after OLAT.
