Change in expression of macrophage migration inhibitory factor mRNA in a rat model of ventilator-induced lung injury
10.3760/cma.j.issn.0254-1416.2013.11.027
- VernacularTitle:呼吸机相关性肺损伤大鼠肺组织巨噬细胞动抑制因子mRNA表达的变化
- Author:
Huijun DAI
;
Linghui PAN
;
Fei LIN
;
Wanyun GE
;
Wei LI
;
Sheng HE
- Publication Type:Journal Article
- Keywords:
Respiration,artificial;
Respiratory distress syndrome,adult;
Macrophage migration-inhibitory factors;
iRNA messenger;
Lung
- From:
Chinese Journal of Anesthesiology
2013;33(11):1386-1388
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes in the expression of macrophage migration inhibitory factor (MIF) mRNA in a rat model of ventilator-induced lung injury.Methods Thirty adult male Sprague-Dawley rats,weighing 2β5-260 g,were randomly divided into 3 groups (n =10 each) using a random number table:control group (group C),small tidal volume (VT) mechanical ventilation group (group S) and large tidal volume mechanical ventilation group (group L).The animals were anesthetized with intraperitoneal ketamine 100 mg/kg,midazolam 0.2 mg/kg and atropine 1.0 mg/kg.The rats were tracheostomized and spontaneous breathing was maintained in group C,while the rats were tracheostomized and mechanically ventilated for 4 h in groups S and L.The tidal volume was 7 ml/kg (group S) or 40 ml/kg (group L),I ∶ E was 1 ∶ 1,RR was 80 bpm and FiO2 was 100％.At 4 h of spontaneous breathing or mechanical ventilation,broncho-alveolar lung lavage fluid (BALF) was collected for determination of the total protein concentration,white blood cell (WBC) counts and concentrations of MIF,IL-6 and IL-1β (by ELISA).Then the rats were sacrificed and the lungs removed for microscopic examination and for determination of wet to dry lung weight ratio (W/D ratio) and expression of MIF mRNA (by RT-PCR).Results Compared with C and S groups,WBC counts,concentrations of total protein,MIF,IL-6 and IL-1β in BALF,and W/D ratio and expression of MIF mRNA in lung tissues were significantly increased in group L (P ＜ 0.05).There was no significant difference in the indexes mentioned above between group C and group S (P ＞ 0.05).The pathological changes occurred in group L.Conclusion The up-regulation of MIF mRNA expression in lung tissues may be involved in the development of ventilator-induced lung injury in rats.
