Expression of microRNA-200b in cholangiocarcinoma and its effect on apoptosis and invasivness of cholangiocarcinoma cells
10.3760/cma.j.issn.1007-8118.2014.02.012
- VernacularTitle:微RNA-200b在胆管癌中的表达及其对癌细胞凋亡与侵袭转移特性的影响
- Author:
Feng PENG
;
Min WANG
;
Jianxin JIANG
;
Rui TIAN
;
Ming SHEN
;
Renyi QIN
- Publication Type:Journal Article
- Keywords:
Bile duct neoplasms;
MicroRNAs;
Neoplasm invasiveness;
Apoptosis
- From:
Chinese Journal of Hepatobiliary Surgery
2014;20(2):123-127
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the role of differentially expressed microRNA (miRNA) in human cholangiocarcinoma and explore their effects on apoptosis and invasiveness of cholangiocarcinoma.Methods The differential expression of miRNA in 3 cholangiocarcinoma patients was detected by miRNA array.The expressions of miR-200a,miR-200b,miR-200c,and miR-141 in human cholangiocarcinoma tissues and normal bile duct tissues were detected by real-time PCR.After transfection with miR-200b mimic,apoptosis and invasiveness of human cholangiocarcinoma cell line QBC939 was evaluated by Annexin-V-FITC dyeing and Transwell assay.Results Comparad with normal bile duct tissues,the number of differential miRNAs in cholangiocarcinoma was 21,including 15 up regulated and 6 down regulated.The expressions of miR200a,miR-200b,miR-200c,and miR-141 in human cholangiocarcinoma tissues were significantly lower than levels in normal bile duct tissues.The invasive ability of QBC939 was decreased after miR-200b mimic transfection.The apoptosis cell number of QBC939 was increased after miR-200b mimic transfection.Conclusion These results indicate that the expression of miRNA is different between cholangiocarcinoma and normal bile duct tissues.Moreover,miR-200a,miR-200b,miR-200c,and miR-141 are likely involved in the invasion and metastasis of cholangiocarcinoma and have potential as a diagnostic and prognostic marker.
