Correlation of cholangiocarcinoma and K-ras gene mutation of bile duct tissues

VernacularTitle:胆管活检组织K-ras基因突变与胆管癌的相关性
Author: Wenjie ZHANG ; Xuefeng WANG ; Jun GU ; Yijing TAO ; Lei CHEN ; Wenjie LYU ; Jianwei BI
Publication Type:Journal Article
Keywords: Bile duct neoplasms; Bile duct diseases; Genes; Mutation
From: Chinese Journal of Hepatobiliary Surgery 2014;20(2):97-100
CountryChina
Language:Chinese
Abstract: Objective To explore the relationship between K-ras gene and cholangiocarcinoma by detecting the K-ras gene mutation of bile duct tissues.Method We studied all the patients who presented to our hospital from June 2010 to June 2012 with stenosis of the bile duct.There were 17 cases of cholangiocarcinoma and 17 cases of benign stenosis.From the DNA extracted from the paraffin tissues,we used the HRM assay for K-ras gene mutation.Result We found that the HRM method and the DNA typing had exactly the same result for the DNA content which confirmed the effectiveness of the HRM assay.Of note,the K-ras mutation rate was found to be significantly higher in the cholangiocarcinoma cases (11/17) when compared with the benign cases (3/17).Conclusion The mutation of the K-ras gene was closely related to cholangiocarcinoma.Our results suggest a new way to diagnosis cholangiocarcinoma.