Comparison of Methods for Extraction and Determination of Pesticide Residue in Chinese Herbal Medicines

VernacularTitle:中药材中农药残留提取方法比较研究
Author: Xiaohui HUANG ; Yan LIU ; Jian ZHANG
Publication Type:Journal Article
Keywords: pesticide residues; accelerated solvent extraction; ultrasonic extraction; soxhlet extraction; Chinese herbal medicines
From: Chinese Journal of Information on Traditional Chinese Medicine 2014;(4):89-92
CountryChina
Language:Chinese
Abstract: Objective To compare the effects of accelerated solvent extraction (ASE), ultrasonic extraction (USE) and soxhlet extraction (SE), by extraction and determination of pesticide residues in Chinese herbal medicines. Methods Pesticide residues of Chinese herbal medicines were extracted by accelerated solvent extraction, ultrasonic extraction and soxhlet extraction, then the extract were cleaned up by sulfonation treatment, alumina neutral-florisil column and gel permeation chromatography (GPC). The extract was separated by HP-5 capillary column and detected by electron-capture detector. Results Extraction efficiency of USE was significantly lower than that of ASE and SE, there was no significant difference between ASE and SE. Pairwise comparison of the recoveries of three purification methods showed no significant difference. Conclusion The extraction efficiency of three methods was ASE>SE>USE. The extraction method should be selected according to the requirement.