Human umbilical cord mesenchymal stem cells are induced in vitro to differentiate into fibroblasts
10.3969/j.issn.2095-4344.2014.10.012
- VernacularTitle:人脐带间充质干细胞体外诱导分化为成纤维细胞
- Author:
Yi YANG
;
Xin LUO
;
Xuefeng JIANG
;
Hanlin SHUAI
;
Hong SONG
;
Jingli ZHANG
;
Jianfa LAN
- Publication Type:Journal Article
- Keywords:
stem cells;
mesenchymal stem cells;
umbilical cord;
fibroblasts
- From:
Chinese Journal of Tissue Engineering Research
2014;(10):1554-1559
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:The umbilical cord mesenchymal stem cells possess multipotent differentiation capacity, but less research focus on its differentiation into fibroblasts.
OBJECTIVE:To investigate the capacity of human umbilical cord mesenchymal stem cells differentiating into fibroblasts.
METHODS:Using adherent method, human umbilical cord mesenchymal stem cells were isolated, and flow cytometric analysis of the surface antigen was performed. Passage 3 cells were selected for osteogenic and
adipogenic differentiation, and cells differentiated into fibroblasts under the induction of basic fibroblast growth factor. RESULTS AND CONCLUSION:Adherent stem cells were stably isolated from the umbilical cord. Human umbilical cord mesenchymal stem cells lowly expressed CD31, CD45, CD40, HLA-DR, but strongly expressed CD29, CD90, CD44, CD105. Oil red O staining showed red droplets were ful of the cytoplasm after adipogenic induction;alizarin red staining showed red calcium nodules after osteogenic induction. After induced by basic fibroblast growth factor, the type I col agen expression was significantly higher than that in the control group. These findings indicate that the adherent human umbilical cord mesenchymal stem cells are reliably isolated with high purity;basic fibroblast growth factor can induce differentiation of umbilical cord mesenchymal stem cells into fibroblasts.
