Hypoxia-inducible factor-1 alpha effects on bone marrow mesenchymal stem cell mobilization in rats with acute myocardial infarction
10.3969/j.issn.2095-4344.2014.10.016
- VernacularTitle:低氧诱导因子1α高表达对急性心肌梗死大鼠骨髓间充质干细胞的动员
- Author:
Jinwei QI
;
Jinglin CHENG
;
Shu ZHOU
;
Jingrong LI
;
Xuexiang LI
;
Qin YANG
;
Hao ZHANG
;
Jun WAN
;
Yulin WANG
;
Lixin ZHANG
;
Yunyun CHEN
;
Xiuxia XI
;
Li YE
;
Qian TANG
;
Feng XU
;
Yang JANG
;
Leyi HU
;
Zeyan LIU
- Publication Type:Journal Article
- Keywords:
stem cells;
mesenchymal stem cells;
hypoxia-inducible factor 1,alpha subunit;
myocardial infarction
- From:
Chinese Journal of Tissue Engineering Research
2014;(10):1579-1584
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Increasing autologous stem cellmobilization is conceived to achieve effectively repair of cardiac ischemic injury. Therefore, it is important to seek a specific and effective mobilization agent. OBJECTIVE:To observe the effects of hypoxia-inducible factor-1α(HIF-1α) on bone marrow mesenchymal stem cellmobilization in myocardial infarction. METHODS:Left anterior descending artery was ligated to establish a rat model of acute myocardial infarction in 90 outbreeding Sprague-Dawley rats, and then the models were randomly divided into three groups. In HIF-1α-antisense oligonucleotide (ASODN) group, HIF-1α-ASODN was infused into the tail vein to restrain the expression of HIF-1αin infarcted ischemic tissue. In HIF-1α-missense oligonucleotide (MSODN) group or control group, an equal volume of HIF-1α-MSODN or saline was injected. RESULTS AND CONCLUSION:After 30 hours and 7 days of modeling, the number of bone marrow mesenchymal stem cells and expression of vascular endothelial growth factor in the peripheral blood of the control group were similar to the HIF-1α-MSODN group, but significantly higher than the HIF-1α-ASODN group. After 7 days of modeling, the expressions of HIF-1αprotein, vascular endothelial growth factor protein and mRNA in the ischemic myocardial tissues of the control group were similar to the HIF-1α-MSODN group, but significantly higher than the HIF-1α-ASODN group. After 7, 14 and 28 days of modeling, the capil ary density in the ischemic myocardial tissues of the control group was similar to the HIF-1α-MSODN group, but significantly higher than the HIF-1α-ASODN group. These findings indicate that after acute myocardial infarction, high expression of HIF-1αexhibits a causal relationship with mobilization of bone marrow mesenchymal stem cells, initiating a series of self-healing process of myocardial tissues.
