HPLC Determination of Ergosterol in Cultured Cordyceps militaris and Natural Cordyceps sinensis
10.3969/j.issn.1005-5304.2014.04.021
- VernacularTitle:RP-HPLC测定人工虫草和天然虫草中麦角甾醇含量
- Author:
Weiwei ZHANG
;
Tao GONG
;
Donghe HAN
;
Yongjun HU
- Publication Type:Journal Article
- Keywords:
ergosterol;
cultured Cordyceps militaris;
preparations of fermental Cordyceps;
Cordyceps sinensis;
HPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(4):67-69
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an HPLC method for determination of content of ergosterol in cultured Cordyceps militaris and natural Cordyceps sinensis. Methods Diamonsil C18(2) column (150 mm×4.6 mm, 5 μm) was used, with methanol-water (98∶2) as mobile phase, column temperature of 25 ℃ , flow rate of 1.0 mL/min, and detection wavelength of 280 nm. Results The linear range of ergosterol was 0.197 7-3.954 9 μg (r=1.000), and avarage recovery rate was 99.51%, RSD was 0.56% (n=9). Conclusion This method is effective, sensitive and accurate with high repeatability and stability, which is helpful for the determination of ergosterol in the cultured Cordyceps and natural Cordyceps.
