Immunoregulation role of adipose mesenchymal stem cells in J774.1 cells
10.3969/j.issn.2095-4344.2014.10.009
- VernacularTitle:脂肪间充质干细胞对J774.1细胞的免疫调控
- Author:
Li BAI
;
Wei ZHANG
;
Chunyan PANG
;
Feifei WEI
;
Yongfu WANG
- Publication Type:Journal Article
- Keywords:
adipose tissue;
mesenchymal stem cells;
macrophages;
coculture techniques;
immunomodulation;
interleukin-6;
tumor necrosis factor-alpha
- From:
Chinese Journal of Tissue Engineering Research
2014;(10):1532-1538
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Recent studies have found that adipose mesenchymal stem cells can inhibit immune responses of T cells, B cells and dendritic cells. But it is unclear whether adipose mesenchymal stem cells are able to regulate the immune responses of macrophages. OBJECTIVE:To observe the expression of interleukin-6 and tumor necrosis factorαin J774.1 cells co-cultured with adipose mesenchymal stem cells co-cultured. METHODS:Adipose mesenchymal stem cells at a density of 2×107 per wel were inoculated into the upper chamber of the transwel and 24-wel plates. Then, J774.1 cells were suspended with cellculture medium containing 1 mg/L lipopolysaccharide and planted into the lower chamber of the transwel and 24-wel plates containing adipose mesenchymal stem cells as co-culture group. Meanwhile, inactive J774.1 cells to lipopolysaccharide-activat J774.1 cells served as negative and positive control groups, respectively. After 48 hours, the J774.1 cells were col ected to extract the RNA samples. Then, mRNA expressions of interleukin-6 and tumor necrosis factorαwere measured by real-time quantitative PCR. RESULTS AND CONCLUSION:Compared with the positive control group, direct-contact co-culture of adipose mesenchymal stem cells and J774.1 cells significantly reduced expressions of interleukin-6 and tumor necrosis factorαin J774.1 cells, but non-contact co-culture only reduced interleukin-6 expression. These findings indicate that adipose mesenchymal stem cells can inhibit the immune response of J774.1 cells activated by lipopolysaccharide.
