Anti-tumor Effect of Tenacissoside H and Influence on Immune Function of Lewis Lung Cancer Mice
10.3969/j.issn.1005-5304.2014.04.020
- VernacularTitle:通关藤苷H对小鼠Lewis肺癌的抑制作用及对荷瘤小鼠免疫功能的影响
- Author:
Wenjing WEI
;
Guanqing ZHANG
;
Shijie LIU
;
Tongxiang LIU
- Publication Type:Journal Article
- Keywords:
tenacissoside H;
Lewis lung cancer;
tumor inhibition rate;
spleen index;
thymus index;
IL-2;
IL-10;
mice
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(4):63-66
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the anti-tumor effect of tenacissoside H on Lewis lung cancer mice, and explore its impacts on immune function of tumor-bearing mice. Methods Mice was injected Lewis lung cancer cells subcutaneously to the right axilla. Thirty successful tumor-bearing mice were randomly divided into model group, the cyclophosphamide (CTX) group and tenacissoside H group, 10 mice in each group. Three days after inoculation, mice were intraperitoneally injected by normal saline, CTX and tenacissoside H respectively every two days, 0.2 mL in each mouse. On the 21st day, the eyeballs were extracted and blood was drawn, tumor tissue, spleen and thymus were taken and weighted to calculate tumor inhibition rate, spleen index and thymus index, and the contents of IL-2 and IL-10 were detected by ELISA. Results The tumor weights of CTX group and tenacissoside H group were lower than that of the model group with significant difference (P<0.05), and the tumor inhibition rates were 54.12%, 25.68%. The thymus index and spleen index of tenacissoside H group increased, but that of CTX group decreased significantly. Compared with the model group, the IL-2 level of tenacissoside H group was significantly increased, while the IL-10 level decreased. Conclusion Tenacissoside H can inhibit growth and metastasis of Lewis lung cancer, regulate the expression of IL-2 and IL-10, and improve the immune function of tumor-bearing mice.
