Comparison of different assays for detection of Chlamydia trachomatis
10.3969/j.issn.1673-4130.2014.05.009
- VernacularTitle:沙眼衣原体感染不同检测方法的比较
- Author:
Kun DU
- Publication Type:Journal Article
- Keywords:
Chlamydia trachomatis;
fluorescent antibody technique,direct;
polymerase chain reaction
- From:
International Journal of Laboratory Medicine
2014;(5):529-530
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the sensitivity and specificity of different assays for detection of Chlamydia trachomatis . Methods Chlamydia trachomatis was determined in samples of cervical secretions from 213 patients with nongonococcal urethritis or genitourinary tract infection by direct immunofluorescence assay (DFA ) ,gold-immunochromatographic assay (ICA ) and real-time fluorescence quantitative polymerase chain reaction (FQ-PCR) .Results Comparing the positive rates of Chlamydia trachoma-tis detected by adopting the DAF ,FQ-PCR and the ICA methods showed the statistical difference (P<0 .05) .The sensitivity and specificity of Chlamydia trachomatis detected by DFA ,ICA and FQ-PCR were 95 .1% ,60 .2% ,97 .3% and 93 .2% ,99 .2% , 99 .3% ,respectively .The sensitivity of the DFA and FQ-PCR methods was higher than that of the ICA method ,difference was sta-tistically significant(P<0 .05) ,The specificity of the ICA and FQ-PCR methods was higher than that of the DFA method ,differ-ence was statistically significant (P<0 .05) .Conclusion The FQ-PCR method has higher sensitivity and higher specificity for the detection of Chlamydia trachomatis and can provide the reliable basis for clinically diagnosing the infection of Chlamydia trachom-atis .The primary medical units is suitable to adopt the ICA method for detecting Chlamydia trachomatis .
