Comparative research on fluorescent quantitation PCR method and gene chips typing method in testing sensitivity of human papilloma virus
10.3969/j.issn.1673-4130.2014.05.012
- VernacularTitle:荧光定量PCR法和基因芯片分型法检测人乳头瘤病毒的敏感度比较研究
- Author:
Xuegan YAO
;
Xinhai LIU
;
Meicai CHEN
- Publication Type:Journal Article
- Keywords:
polymerase chain reaction;
papillomaviridae;
cytological techniques
- From:
International Journal of Laboratory Medicine
2014;(5):536-537
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the sensitivity of the fluorescent quantitation PCR method (fluorescence quantitative method for short)and the gene chips typing method (gene chips method for short)in testing human papilloma virus(HPV) .Methods 305 outpatients in the gynecological clinic of the hospital from February 2010 to February 2013 were selected as the research subject . Then the cervical exfoliated cells were collected and detected HPV by the fluorescence quantitative method and the gene chips meth-od respectively .Those cases with inconsistent results were confirmed by the PCR sequencing method .305 cases were performed the liquid based cytology test .Results The sensitivity of the fluorescence quantitative method in detecting HPV was 33 .8% and which of the gene chips method was 37 .6% .The two methods had a higher conformance .The HPV infection rate was increased with the increase of cervical exfoliated cell atypia .Conclusion The fluorescence quantitative method and the gene chips method have a higher conformance .The sensitivity of the gene chips method is higher .The severity degree of cervical cytological change is positively cor-related with the HPV infection .
