Study about effects of BCG treatment on expression of stem cell factor in asthmatic mice
10.3969/j.issn.1000-3606.2014.03.019
- VernacularTitle:卡介苗干预对哮喘小鼠干细胞因子表达的影响
- Author:
Xiaoliang LIN
;
Jianhua ZHANG
- Publication Type:Journal Article
- Keywords:
asthma;
eosinophils;
stem cell factor;
Bacillus Calmette-Guerin
- From:
Journal of Clinical Pediatrics
2014;(3):261-264
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of Bacillus Calmette-Guerin (BCG) intervention on the expression of stem cell factor (SCF) in asthma. Methods Kunming mice were randomly divided into asthmatic group, BCG group and control group of ten mice each group. Mice were sensitized and challenged with ovalbumin (OVA) to establish asthmatic model. After twenty-four hours of last challenge, eosinophils (EOS) were counted in the lung tissue and bronchoaveolar lavage fluid (BALF). The level of SCF in BALF was determined by enzyme-linked immunosorbent assay. The expression of SCF protein in lung tis-sue was measured by immunohistochemistry technique and computerized image analysis system. Results The number of EOS in lung tissue was (10.67±1.94)/HP, (6.40±1.55)/HP and (0.37±0.33)/HP in asthmatic, BCG and control group respectively. The number of EOS in BALF was (7.58 ± 1.30) × 107/L, (3.78 ± 1.15) × 107/L and 0 in asthmatic, BCG and control group respectively. The difference between each group was statistically significant (P<0.01). The immunohistochemical index of SCF protein in lung was (24787.97±7214.12), (20509.50±4775.27) and (12261.66±3277.65) in asthmatic, BCG and control group respectively. The SCF level of BALF was (280.25 ± 14.20) pg/ml, (266.77 ± 31.15) pg/ml and (223.59 ± 15.61) pg/ml in asthmatic, BCG and control group respectively. The SCF level in BALF in asthmatic and BCG group was significantly different from that in control group (P<0.05). There was no significant difference of SCF level in BALF between BCG and asthmatic group (P>0.05). In asth-matic group, the expression of SCF in lung was positively correlated with the number of EOS (P<0.05). Conclusions BCG treat-ment can markedly decrease the airway inflammation in asthmatic mice. BCG cannot inhibit the expression of SCF in asthma.
