Detection and clinical significance of peripheral blood CD 4+T cell-derived leptin in patients with Hashimoto′s thyroiditis
10.3969/j.issn.1673-4130.2014.05.010
- VernacularTitle:桥本甲状腺炎患者外周血CD4＋T 细胞瘦素检测及临床意义
- Author:
Qinglei XU
;
Baolin ZHU
;
Xiaobo MA
;
Wei ZHANG
;
Lingling LIU
;
Shengjun WANG
;
Jianguo CHEN
- Publication Type:Journal Article
- Keywords:
thyroiditis,autoimmune;
leptin;
enzyme-linked immunosorbent assay
- From:
International Journal of Laboratory Medicine
2014;(5):531-532
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the level of CD4+ T cell-derived leptin(LP) in peripheral blood mononuclear cells(PBMC) from the patients with Hashimoto′s thyroiditis(HT) and to investigate its role in the occurrence and development of HT .Methods The peripheral blood samples in the patients with HT and the healthy controls were collected and separated for obtaining PBMC .The CD4+ T cells magnetic beads was adopted to separate CD4+ T cells for culturing in vitro .The LP level in supernatant was detected by ELISA .The relative expression amount of the orphan nuclear receptor RORγt was detected by real-time quantitative PCR (qRT-PCR) method .Results The LP level of CD4+ T cell culture liquid in the HT patients was markedly higher than that in the healthy control group ,difference was statistically significant (P<0 .05) .Furthermore ,the LP level in CD4+ T cell culture liquid was posi-tively related with the relative expression amount of RORγt in HT patients .Conclusion The CD4+ T cell-induced leptin level is in-creased in HT patients ,which is closely related with the occurrence and development of HT .
