Methodological and Applicability Study of Rat Skin Anaphylactoid Test
10.3969/j.issn.1005-5304.2014.02.019
- VernacularTitle:大鼠皮肤类过敏试验的方法学研究及适用性考察
- Author:
Libo ZHANG
;
Jiajun XIE
;
Juanjuan YI
;
Lijing KANG
;
Lin ZHAO
- Publication Type:Journal Article
- Keywords:
rat skin test;
anaphylactoid reaction;
methodological studies;
C48/80;
endotoxin;
Xuesetong injection;
Tanreqing injection
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(2):67-71
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the sensitivity and reliability of rat skin anaphylactoid test method, detect and evaluate the anaphylactoid reaction of traditional Chinese medecine injection. Methods The condition of rat skin anaphylactoid test was optimized by studying the influencing factors of sensitivity and reliability of test with C48/80 as a tool drug, Tween80, endotoxin, China cobra toxin, trichosanthin injection, Tanreqing injection and Xuesaitong injection were investigated. Results The best conditions of rat skin anaphylactoid test was as follows:intrademal inject the drug with insulin syringe, 50-100μL per point, immediately inject 0.5%Evans blue dye 1 mL though caudal vein, 15 min later, kill the rat by carotid artery bleeding, clip dorsal skin to do the test. With this method, Tween80, endotoxin, China cobra toxin and trichosanthin injection all can induce blue stain in rat skin. Tanreqing injection showed no blue stain at the clinical dose. Xuesaitong injection although can induce blue stain in rat skin at the clinical dose, but the results cannot exclude the interference of its pharmacological function. Conclusion The method is simple with short test cycle, less dose of test drug, high detection sensitivity and good reproducibility, but some drug can show false positive result due to its own property.
