Autophagy-related gene Beclin-1 expression in neuron-like differentiation of human bone marrow mesenchymal stem cells
10.3969/j.issn.2095-4344.2014.06.004
- VernacularTitle:人骨髓间充质干细胞神经元样细胞分化中自噬相关基因Beclin-1的表达
- Author:
Yi YANG
;
Wenjing DING
;
Wanli DONG
- Publication Type:Journal Article
- Keywords:
autophagy;
celldifferentiation;
mesenchymal stem cells;
glial fibril ary acidic protein;
neurons
- From:
Chinese Journal of Tissue Engineering Research
2014;(6):841-846
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Bone marrow mesenchymal stem cells have potential to self-renewal and multi-lineage differentiation. But after a long period of culture in vitro, the proliferation and differentiation capacities of bone marrow mesenchymal stem cells gradual y loss, the mechanism underlying which is not clear now.
OBJECTIVE:To observe the expression of autophagy-related gene Beclin-1 in differentiation from human bone marrow mesenchymal stem cells into neuron-like cells in vitro.
METHODS:The changes of morphological characteristics of neuron-like cells differentiated from human bone marrow mesenchymal stem cells induced by epidermal growth factor were observed. The expression of neuron-specific enolase and glial fibril ary acidic protein in treated and untreated human bone marrow mesenchymal stem cells were detected using immunocytochemistry. The Beclin-1 protein expressions were detected by western blot before and after induction.
RESULTS AND CONCLUSION:After being induced, human bone marrow mesenchymal stem cells presented classical neuron-like morphology;the expressions of neuron-specific enolase and glial fibril ary acidic protein were 78.7%and 8.1%, respectively. The expression of Beclin-1 protein was changed correspondingly during the induction, which increased after 30 minutes of induction and decreased gradual y after 1 hour of induction. Human bone marrow mesenchymal stem cells could be induced into neuron-like cells in vitro by epidermal growth factor. Autophagy-related gene was highly expressed in the induction of early differentiation and the expression gradual y reduced until it remained at a low level during the differentiation.
